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Microbial
Symbiosis

Faculty & Staff

Margaret McFall-Ngai

General Information

Professor
Squid-Vibrio mutualism

Office: Department of Medical Microbiology & Immunology
5245 Microbial Sciences Building
1550 Linden Drive
Madison, WI 53706

Phone: (608) 262-2393
Email: mjmcfallngai@wisc.edu

Academic Background

B.S. Biology University of San Francisco 1973
Ph.D. Biology UCLA 1983
Postdoc Jules Stein Eye Institute-UCLA 1984-86
Postdoc Scripps Institution of Oceanography UC San Diego 1986-88

Summary of Research

Symbiotic associations between animals and prokaryotes:

The ‘design’ of tissues that interact with light:

Publications

2011

Attenuation of host NO production by MAMPs potentiates development of the host in the squid-vibrio symbiosis. Cell Microbiol. 2011 Apr;13(4):527-37. Altura MA, Stabb E, Goldman W, Apicella M, McFall-Ngai MJ.

2010

Vibrio fischeri flavohaemoglobin protects against nitric oxide during initiation of the squid-Vibrio symbiosis. Mol Microbiol. 2010 Nov;78(4):903-15). Wang Y, Dunn AK, Wilnett J, McFall-Ngai MJ, Spiro S, Ruby EG.

A New Design for the ASM General Meeeting. MBio. 2010 Nov 23;1(5). Miller JF, McFall-Ngai MJ, Casadevall A.

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis. Proc Natl Acad Sci USA. 2010 Feb 2;107(5):2259-64. Wier AM, Nyholm SV, Mandel MJ, Massengo-Tiassé RP, Schaefer AL, Koroleva I, Splinter-Bondurant S, Brown B, Manzella L, Snir E, Almabrazi H, Scheetz TE, Bonaldo Mde F, Casavant TL, Soares MB, Cronan JE, Reed JL, Ruby EG, McFall-Ngai MJ.

Taming of the symbiont for coexistence: a host PGRP neutralizes a bacterial symbiont toxin. Troll JV, Bent EH, Pacquette N, Wier AM, Goldman WE, Silverman N, McFall-Ngai MJ. Environ Microbiol. 2009 Dec 27.

2009

Whole-mount in situ hybridization of Hawaiian bobtail squid (Euprymna scolopes) embryos with DIG-labeled riboprobes: II. Embryo preparation, hybridization, washes, and immunohistochemistry. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot5322.

Whole-mount in situ hybridization of Hawaiian bobtail squid (Euprymna scolopes) embryos with DIG-labeled riboprobes: I. DNA template preparation and in vitro transcription of riboprobes. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot5321.

Confocal immunocytochemistry of embryonic and juvenile Hawaiian bobtail squid (Euprymna scolopes) tissues. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot5320.

Preparation of genomic DNA from Hawaiian bobtail squid (Euprymna scolopes) tissue by cesium chloride gradient centrifugation. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.prot5319.

The Hawaiian bobtail squid (Euprymna scolopes): a model to study the molecular basis of eukaryote-prokaryote mutualism and the development and evolution of morphological novelties in cephalopods. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. Cold Spring Harb Protoc. 2009 Nov;2009(11):pdb.emo135.

Evidence for light perception in a bioluminescent organ. Tong D, Rozas NS, Oakley TH, Mitchell J, Colley NJ, McFall-Ngai MJ. PNAS. 2009 June 9.

Dr. Margaret McFall-Ngai interviewed on NPR Canada discussing the convergence of eye and light organ : Click here to listen

Peptidoglycan induces loss of a nuclear peptidoglycan recognition protein during host tissue development in a beneficial animal-bacterial symbiosis. Troll JV, Adin DM, Wier AM, Paquette N, Silverman N, Goldman WE, Stadermann FJ, Stabb EV, McFall-Ngai MJ. Cell Microbiol. 2009 Mar 26.

Recognition between symbiotic Vibrio fischeri and the haemocytes of Euprymna scolopes. Nyholm SV, Stewart JJ, Ruby EG, McFall-Ngai MJ. Environ Microbiol. 2009 Feb;11(2):483-93.

2008

Mutations in ampG and lytic transglycosylase genes affect the net release of peptidoglycan monomers from Vibrio fischeri. Adin DM, Engle JT, Goldman WE, McFall-Ngai MJ, Stabb EV. J Bacteriol. 2008 Dec 12.

Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association.Chun CK, Troll JV, Koroleva I, Brown B, Manzella L, Snir E, Almabrazi H, Scheetz TE, Bonaldo Mde F, Casavant TL, Soares MB, Ruby EG, McFall-Ngai MJ. Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11323-8. Epub 2008 Aug 5.

2007

Adin DM, Phillips NJ, Gibson BW, Apicella MA, Ruby EG, McFall-Ngai MJ, Hall DB, Stabb EV. Characterization of htrB and msbB mutants of the light organ symbiont Vibrio fischeri. Appl Environ Microbiol. 2007 Dec 7.

Koropatnick, Kimbell, and Mcfall-Ngai. Responses of hosthemocytes during the initiation of the squid-Vibrio symbiosis. Biol. Bull. 2007 Feb; 212:29-39.

McFall-Ngai M. Adaptive immunity: care for the community. Nature. 2007 Jan 11;445(7124):153.

McFall-Ngai M. Love the one you're with: vertebrate guts shape their microbiota. Cell. 2006 Oct 20;127(2):247-9.

2006

Goodson MS, Crookes-Goodson WJ, Kimbell JR, McFall-Ngai MJ. Characterization and role of p53 family members in the symbiont-induced morphogenesis of the Euprymna scolopes light organ. Biol Bull. 2006 Aug;211(1):7-17.

Cloud-Hansen KA, Peterson SB, Stabb EV, Goldman WE, McFall-Ngai MJ, Handelsman J. Breaching the great wall: peptidoglycan and microbial interactions. Nat Rev Microbiol. 2006 Sep;4(9):710-6.

Chun CK, Scheetz TE, Bonaldo MF, Brown B, Clemens A, Crookes-Goodson WJ, Crouch K, Demartini T, Eyestone M, Goodson MS, Janssens B, Kimbell JL, Koropatnick TA, Kucaba T, Smith C, Stewart JJ, Tong D, Troll JV, Webster S, Winhall-Rice J, Yap C, Casavant TL, McFall-Ngai MJ, Soares MB. An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri. BMC Genomics. 2006 Jun 16;7(1):154.

Sycuro LK, Ruby EG, McFall-Ngai M. Confocal microscopy of the light organ crypts in juvenile Euprymna scolopes reveals their morphological complexity and dynamic function in symbiosis. J Morphol. 2006 Jan 20.

2005

Goodson MS, Kojadinovic M, Troll JV, Scheetz TE, Casavant TL, Soares MB, McFall-Ngai MJ. Identifying components of the NF-kappaB pathway in the beneficial Euprymna scolopes-Vibrio fischeri light organ symbiosis. Appl Environ Microbiol. 2005 Nov;71(11):6934-46.

 

University of Wisconsin-Madison
1550 Linden Drive
Rm 1336 Dept of Medmicro
Madison, WI 53706-1521

Phone: (608) 890-2101
Fax: (608) 262-8418

Email: info@microbes.wisc.edu